Point-of-Care Testing (POCT) Certificate 2025 – 400 Free Practice Questions to Pass the Exam

A competitive assay is a specific type of immunoassay used to quantify the concentration of an analyte, which could be a hormone, drug, or other biomolecules. This method works by introducing a known amount of labeled analyte that competes with the analyte present in a sample for binding sites on a specific antibody. The more analyte present in the sample, the less labeled analyte binds to the antibody, allowing for a quantitative measure of the analyte's concentration based on how much signal (often in terms of fluorescence or color change) remains.

The purpose of competitive assays is to derive the concentration of the analyte in the test sample by comparing the binding of the analyte in the sample with that of a reference standard, which is directly related to its concentration. This is why the option referring to analyte concentration is the correct answer, as competitive assays are designed specifically to measure that.

On the other hand, the pathways that involve multiple pathogens, antibody presence, and color intensity, while related to immunoassays, do not inherently define the purpose of a competitive assay. In competitive assays, the primary focus is not on identifying multiple pathogens or solely detecting antibodies. Color intensity, while an observable result of the assay,